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Moreover, colocalization of the ROMK protein and a mitochondrial marker in the mitochondria of fibroblast cells was shown by immunofluorescence. The presence of ROMK protein was observed in the inner mitochondrial membrane fraction. Using reverse transcriptase-PCR, we found an mRNA transcript for the KCNJ1(ROMK) channel. Additionally, the potassium channel opener diazoxide lowered the amount of superoxide formed in isolated fibroblast mitochondria. The influence of substances modulating ATP-regulated potassium channel activity on oxygen consumption and membrane potential of isolated fibroblast mitochondria was also studied. Channel activity was inhibited by antidiabetic sulfonylurea glibenclamide and 5-hydroxydecanoic acid. The activity of this channel was inhibited by a complex of ATP/Mg2+ and activated by potassium channel openers such as diazoxide or BMS 191095. Using the patch-clamp technique on the inner mitochondrial membrane of fibroblasts, we detected a potassium channel with a mean conductance equal to 100 pS in symmetric 150 mM KCl. In the present study, we show for the first time mitochondrial ATP-regulated potassium channels in human dermal fibroblast cells. 309-318 ISSN: 0005-2728 Subject: cell lines, diazoxide, fibroblasts, fluorescent antibody technique, glibenclamide, humans, membrane potential, messenger RNA, mitochondria, mitochondrial membrane, oxygen consumption, patch-clamp technique, potassium channels, potassium chloride, reverse transcriptase polymerase chain reaction Abstract: Mitochondrial ATP-regulated potassium channels are present in the inner membrane of the mitochondria of various cells. Evidence for a mitochondrial ATP-regulated potassium channel in human dermal fibroblasts Author: Piotr Bednarczyk, Anna Kicinska, Michal Laskowski, Bogusz Kulawiak, Rafal Kampa, Agnieszka Walewska, Milena Krajewska, Wieslawa Jarmuszkiewicz, Adam Szewczyk Source: Biochimica et biophysica acta 2018 v.1859 no.5 pp.